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Avengers Age of Ultron 2015 720p Esub BluRay 5. ΔG = ΔG BN + RT ln For ATP + H2O ADP + Pi then ΔG = ΔG BN + RT ln Thus: ATP + H2O ADP + Pi ΔG BN= -30 kJmol-1 How does this compare to the standard free energy change?.Calculate the free energy change for the hydrolysis of ATP in this cell (at 25 oC and otherwise standard conditions) In a rat hepatocyte, the concentrations of ATP, ADP + Pi are 3.4mM, 1.3mM and 4.8mM respectively.Therefore sufficient free energy to support synthesis of ATP!.NOTE: ADP + Pi ATP + H2O ΔG BN= +30 kJmol-1.ΔEON ΔEON found from (half reaction with more positive EON) - (half reaction with less positive EON) ΔG BN = -nFΔEON NOTE: F (Faraday’s constant) 96.48kJV-1mol-1 = (-2)(96.48kJV-1mol-1)(0.26V) = -50.17 kJmol-1 Show that the oxidation of FADH2 by Q theoretically liberates enough energy to drive the synthesis of ATP from ADP and Pi under standard conditions.The standard reduction potential for ubiquinone (Q) is + 0.04V and the standard reduction potential for FAD is -0.22V.NOTE: also phosphoenolpyruvate and 1,3 -bisphosphoglycerate when hydrolysed will provide sufficient free energy.Therefore:reaction exergonic and spontaneous, so in this case YES! The approach here is to determine whether the net free energy change for a coupled reaction is negativeĮg ΔG BN (kJ mol-1 ) ADP + Pi ATP + H2O +30 Phosphocreatine + H2O creatine + Pi -43 - Phosphocreatine + ADP º creatine + ATP -13.MetaboliteΔG BN hydrolysis (kJ mol-1 ).Could any of the following be used as an energy-rich metabolite for replenishment of cellular ATP from ADP? …ie (3) reversed Sucrose + Pi glucose 1-phosphate + fructose …………….(1) ΔG BN = -8 kJ mol-1 or –8000Jī) to calculate the equilibrium constant (Keq) glucose + Pi H2O + glucose 1-phosphate.add the ΔG BN values of the two reactions which combine to yield reaction (1).and b) calculate the equilibrium constant for the phosphorolysis of sucroseĪ1: To calculate a)the biochemical standard free energy ( ΔG BN) for the phosphorolysis of sucrose,.H2O + glucose 1-phosphate glucose + Pi.…(1)Ī) From the following data, calculate the biochemical standard free energy change for the phosphorolysis of sucrose
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Thermodynamics II:problems Dr Una Fairbrother